AOAC SPIFAN Nutrients ERP Report (December 7, 2022)

REVIEW OF METHODS: BIOTIN

CODIFICATION: 2016.02 METHOD TITLE: Determination of biotin by UPLC coupled with Immunoaffinity Column clean-up extraction REVIEWERS: Estela Kneeteman & Karen Schimpf VOTE: Motion – No action to be taken by the ERP for this method. The ERP will consider this method in a future submission as a “new” method Estela Kneeteman motioned Karen Schimpf second Vote: 9 Yes/0 No/1 Abstain

RECOMMENDATION/COMMENTS: 1. Additional recommendations 2. See data on the goat matrices

3. Samples prepared with the enzymes 4. Need to see actual spiked recovery 5. Bias against the current method 6. See where it eludes (Biocytin in the UPLC chromatography)

Motion to approve recommendations: Brendon Gill motioned Tom Vennard second Unanimous