AOAC SPIFAN Nutrients ERP Report (December 7, 2022)

Motion to approve recommendations: Yannis Vrasidas motioned Philip Haselberger second Abstain – Société des Produits Nestlé

CODIFICATION: HMO-04 METHOD TITLE: Validation of an LC-MS method for the analysis of milk oligosaccharides

REVIEWERS: Philip Haselberger & Yannis Vrasidas VOTE: Motion - ERP takes no action on this method Yannis Vrasidas motioned Philip Haselberger second Vote: 10 Yes/0 No/0 Abstain RECOMMENDATION/COMMENTS: 1. No further action.

Motion to approve recommendations: No action taken

CODIFICATION: HMO-05 METHOD TITLE: Method for the Determination of 2’-Fucosyllactose (2’-FL) , 3-Fucosyllactose (3-FL) , 6’-Sialyllactose (6’-SL), 3’-Sialyllactose (3’-SL), Lacto-N-Tetraose (LNT), and Lacto-N-neoTetraose (LNnT) by High Performance Anion Exchange Chromatography, with Pulsed Amperometric Detection (HPAEC-PAD)

REVIEWERS: Philip Haselberger & Yannis Vrasidas VOTE: Motion - To recommended method for First Action Official Methods SM status Sean Austin motioned Brendon Gill second Vote: 9 Yes/0 No/1 Abstain RECOMMENDATION/COMMENTS: 1. Do not include matrices fortified with DFL (out of scope) 2. Update on references in the method 3. Mixture of Salt form with free form (for clarification) a. Consider alternative of using nitrogen instead of helium (or both)

Motion to approve recommendations: Sean Austin motioned Brendon Gill second Abstain – Abbott Nutrition