AOAC SPIFAN Nutrients ERP Report (July 25, 2023)

AOAC Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN)

Maryland

NUTRIENTS EXPERT REVIEW PANEL (ERP) REPORT

Tuesday, July 25, 2023

AOAC INTERNATIONAL 2275 Research Blvd., Suite 300 Rockville, MD, 20850 USA

dboyd@aoac.org 301.924.7077 x126

AOAC Stakeholder Program on Infant Formula & Adult Nutritionals (SPIFAN) Nutrients Expert Review Panel (ERP)

The AOAC SPIFAN Nutrients Expert Review Panel (ERP) Chair confirms the following ERP Report has been reviewed and approves the final version of the Official Chair’s Report for Nutrients July 25, 2023

______________________ Signature ______________________ Date

AOAC INTERNATIONAL Stakeholder Program on Infant Formula & Adult Nutritionals (SPIFAN) Meeting at AOAC INTERNATIONAL & Virtually 2275 Research Blvd. Suite 300, Rockville, MD, USA Tuesday, July 25, 2023 REPORT OF THE NUTRIENTS EXPERT REVIEW PANEL (ERP) PROCEEDINGS Expert Review Panel Members (in attendance):

Darryl Sullivan

Eurofins Food Integrity & Innovation (Chair)

Sean Austin Esther Campos-Giménez Christophe Fuerer

Société des Produits Nestlé S.A.

Sneh Bhandari Hans Cruijsen Martine van Gool Brendon Gill

SDB Consultants FrieslandCampina

Fonterra Cooperative

Jonathan Draher Philip Haselberger (HMO ONLY) Karen Schimpf

Abbott Nutrition

Melissa Phillips Kate Rimmer Shay Phillips

NIST

Reckitt

Thomas Vennard Yannis Vrasidas (HMO ONLY)

Independent Consultant

Jinchuan Yang

Waters Corp.

Expert Review Panel Members (in attendance):

Maria Ofitserova David Woollard

Pickering Lab

Independent Consultant

AOAC Staff Includes: Delia Boyd Pam Coleman Deborah McKenzie Alicia Meiklejohn Observers: Scott Bloomer, AOCS Simone Carron, MXNS Christine Chapman, Hygiena David Ellingson, Eurofins Amy Erdman

Renee Erney, Abbott Nutrition Jennifer Frueh, Reckitt Corp. Micah Greenzweig, Hygiena (New Castle, DE)

Pierre-Alain Golay, Nestlé Research Francesca Giuffrida, Nestlé Research Daniëlla Holt, FrieslandCampina

Harvey Indyk, Fonterra Cooperative Group Ltd George Joseph, AsureQuality New Zealand Erik Konings, Société des Produits Nestlé S.A. Deja Latney, Hygiena Márton Lengyel, Glycom-DSM Peter Sanders, Eurofins Dustin Starkey, Abbott Nutrition Karla Steele, Reckitt Geralt ten Kate, FrieslandCampina Jeroen van Soest, Eurofins CCC Paul Wehling, ChemStats Consulting

I. WELCOME AND INTRODUCTIONS Darryl Sullivan welcomed all participants to the ERP meeting and the ERP members were introduced along with roll call. II. REVIEW OF METHODS BY EXPERT REVIEW PANEL (ERP) FOR FIRST ACTION OFFICIAL METHOD SM STATUS For each method, the ERP reviewed and discussed method(s) submitted for First Action Official Method SM status.

Method Title

Reviewer(s)

Vote

Method

Comments

 To add system suitability criteria on the resolution of the critical pair of the LNT & LNnT

HMO-06 – AOAC Official Method 20xx.xx Analysis of Eight Human Milk Oligosaccharides (HMOs) in Infant Formula and Premix Samples by Labelling with Benzocaine and Analysis by HPLC-UV First Action 20xx

HMO

Philip Haselberger Sean Austin

Motion: To recommend no action for HMO-06 Sean Austin moved & Philip Haselberger Second Unanimous

Method Title

Reviewer(s)

Vote

Method

Comments

SN2-02 - Animal and vegetable fats and oils and Milk and milk products—Fatty acids at the sn-2 position of triacylglycerol molecules- Enzymatic transesterification method

 Data converted to acceptable units  AOAC format  System suitability needs to be added o Suitability of the enzymes being used o Suitability around the chromatography  Clarify the references to other AOCS methods: 2-ISO standard/1 AOCS standard given as references  Addi�onal precision data needs to be generated prior to final ac�on considera�on

FATTY ACIDS AT THE SN-2 POSITION OF TRIACYLGLYCEROL MOLECULES

Brendon Gill Jonathan Draher

Motion: To move the candidate method SN2-02 for First Action Official Methods SM status Jonathan Draher moved & Sneh Bhandari Second Yes 4/No 2/ Abstain 3 2nd vote Yes 5/No 2/ Abstain 3 Motion to recommend list be submitted to method author along with reviewer information Sneh Bhandari moved & Tom Vennard second Unanimous

III. REVIEW OF METHODS BY EXPERT REVIEW PANEL (ERP) FOR FINAL ACTION OFFICIAL METHOD SM STATUS For each method, the ERP reviewed and discussed method(s) submitted for Final Action Official Method SM status.

Method Title

Reviewer(s)

Vote

Method

Comments

2021.01- (GOS-03) - Determination β Galactooligosaccharides (GOS) in Infant Formula and Adult Nutritionals: Collaborative Study

 Need clarifica�on of Annex B

β GALACTOOLIGOSACC HARIDES (GOS)

Estela Kneeteman Hans Cruijsen

Motion: To recommended

method for Final Action Official Methods SM status for infant formula only Estela Kneeteman moved & Hans Cruijsen Second Unanimous Nestle Abstained

Method Title

Reviewer(s)

Vote

Method

Comments

Maria Ofitserova Tom Vennard

2021.07 - (Lacto-01) - Multi-Laboratory Validation of a Biosensor Immunoassay Method for the Analysis of Bovine Lactoferrin in Infant Formula and Adult Nutritionals

 Single data point be included in the final publica�on  In scope sec�on, include other matrices were included in the SLV

LACTOFERRIN

Motion: To recommend the method 2021.07 to Official Methods SM Final Action status Melissa Phillips moved & Sneh Bhandari second Yes 4/No 2/ Abstain 3 2nd Motion: To recommend the method 2021.07 to Official Methods SM Final Action status for infant formula containing intact bovine milk protein Tom Vennard moved & Martine van Gool second Unanimous Fonterra abstained

IV.

NEXT STEPS/FEEDBACK FROM EXPERT REVIEW PANEL Darryl Sullivan provided next steps including recommendations from the Expert Review Panel to method authors along with future dates for AOAC Analytical Methods Week currently slated for November 13-17, 2023. The AOAC SPIFAN Nutrients ERP meeting date will be communicated prior to the meeting.