AOAC SPIFAN Nutrients ERP Report (July 25, 2023)

I. WELCOME AND INTRODUCTIONS Darryl Sullivan welcomed all participants to the ERP meeting and the ERP members were introduced along with roll call. II. REVIEW OF METHODS BY EXPERT REVIEW PANEL (ERP) FOR FIRST ACTION OFFICIAL METHOD SM STATUS For each method, the ERP reviewed and discussed method(s) submitted for First Action Official Method SM status.

Method Title

Reviewer(s)

Vote

Method

Comments

 To add system suitability criteria on the resolution of the critical pair of the LNT & LNnT

HMO-06 – AOAC Official Method 20xx.xx Analysis of Eight Human Milk Oligosaccharides (HMOs) in Infant Formula and Premix Samples by Labelling with Benzocaine and Analysis by HPLC-UV First Action 20xx

HMO

Philip Haselberger Sean Austin

Motion: To recommend no action for HMO-06 Sean Austin moved & Philip Haselberger Second Unanimous

Method Title

Reviewer(s)

Vote

Method

Comments

SN2-02 - Animal and vegetable fats and oils and Milk and milk products—Fatty acids at the sn-2 position of triacylglycerol molecules- Enzymatic transesterification method

 Data converted to acceptable units  AOAC format  System suitability needs to be added o Suitability of the enzymes being used o Suitability around the chromatography  Clarify the references to other AOCS methods: 2-ISO standard/1 AOCS standard given as references  Addi�onal precision data needs to be generated prior to final ac�on considera�on

FATTY ACIDS AT THE SN-2 POSITION OF TRIACYLGLYCEROL MOLECULES

Brendon Gill Jonathan Draher

Motion: To move the candidate method SN2-02 for First Action Official Methods SM status Jonathan Draher moved & Sneh Bhandari Second Yes 4/No 2/ Abstain 3 2nd vote Yes 5/No 2/ Abstain 3 Motion to recommend list be submitted to method author along with reviewer information Sneh Bhandari moved & Tom Vennard second Unanimous