AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

B. Apparatus Note: Carotenoids are light sensitive and all work should be done under protection from UV light. (a) UHPLC system .—Consisting of binary pump, autosampler, thermostatted column compartment, UV-Vis detector, and data acquisition software. Note : Although initial validation was done with a 60 mm flow cell, the method also performs well with a standard 10 mm cell. (b) Analytical column .—C30 Carotenoid column, 3 μm, 2.0 x 250 mm (Part No. CT99S03- 2502WT; YMC, Kyoto, Japan). Other columns may be used if the system suitability criteria in G(b) can be met. (c) Guard column .—C30 Guard column, 3 μm, 2.1 x 10 mm (Part No. CT99S03-01Q1GC; YMC). (d) Guard cartridge holder .— Part No. XPGCH-Q1 (YMC). (e) Spectrophotometer .—Wavelength range of 200-700 nm, with 1 cm quartz cells.

(f) Top loading balance .—Capable of weighing to 0.1 g. (g) Analytical balance .—Capable of weighing to 0.01 mg. (h) Ultrasonic water bath .—40 kHz.

(i) Reciprocating shaker .—Capable of 200 rpm. (j) Evaporator .—With pure nitrogen supply. (k) Laboratory centrifuge .—With adapters for 50 mL centrifuge tubes. (l) Centrifuge tubes .—50 mL, polypropylene. (m) Syringes .—1 mL, disposable.

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