AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

(n) Syringe filters .—0.2 μm, PTFE. (o) Class A volumetric flasks .—Various sizes, clear and amber. (p) Scintillation vials .—12 mL, amber.

(q) HPLC vials .—amber, with 300 μL inserts. (r) Class A volumetric pipets .—Various sizes. C. Reagents Note: Reagent volumes may be scaled up or down provided good laboratory practices are followed. (a) Laboratory water .—>18 megaohm-cm. (b) Methanol (MeOH) .—HPLC grade. (c) Methyl tert-butyl ether (MTBE) .—HPLC grade. (d) n-Hexane .—HPLC grade. (e) Potassium hydroxide (KOH) .—pellets, ACS grade. (f) Reagent alcohol (ROH) .—denatured ethanol, HPLC grade. (g) α-Tocopherol (Vitamin E) .—synthetic, >95%; Acros Organics Part No. 428121000, Sigma Part No. T3251, or equivalent. (h) Pyrogallic acid (Pyrogallol) .—ACS grade. (i) 2-Propanol (IPA) .—HPLC grade. (j) Tetrahydrofuran (THF) .—99.9%, stabilized with BHT. (k) Ammonium acetate .—HPLC grade, 98%. (l) Potassium hydroxide solution, 50% (w/w).— Add 50 mL water to a 250 mL beaker. Weigh 50 g KOH and slowly transfer to the beaker under constant stirring. When

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