AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

dissolved and cooled, transfer to a media bottle and store at room temperature up to 6 months. (m) MTBE/Vitamin E (10 mM).— Dissolve 1.1 g α-tocopherol in 250 mL MTBE. Store in refrigerator up to 1 month. (n) THF/Vitamin E (10 mM).— Dissolve 1.1 g α-tocopherol in 250 mL THF. Store in refrigerator up to 1 month. (o) Pyrogallol solution (0.2 M pyrogallic acid in ethanol).— Dissolve 6.3 g pyrogallic acid in 250 mL ROH. Store in refrigerator up to 1 month. Solution should be clear at room temperature; discard if colored. (p) Extraction solution [1 mM vitamin E in MTBE-THF (1 + 1)].— Combine 50 mL THF/Vitamin E, C(n) , with 250 mL MTBE and 200 mL THF. Store in refrigerator up to 1 month. (q) Sample solvent (10 mM vitamin E in IPA).— Dissolve 3.3 g α-tocopherol in 750 mL IPA. Store in refrigerator up to 1 month. (r) Mobile phase for LC system.—(1) Phase A .—20 mM ammonium acetate in methanol– water (98 + 2). Combine 980 mL MeOH, 20 mL water, and 1.54 g ammonium acetate and mix to dissolve. (2) Phase B .—MTBE. D. Standards (a) Lutein .—ChromaDex (Irvine, CA) Part No. ASB-00012453 or equivalent. (b) β-Carotene .—USP (Rockville, MD) Part No. 1065480 or equivalent. (c) Apocarotenal (β-Apo-8′-carotenal) .—USP Part No. 1040854 or equivalent. (d) Lycopene, >90% by UV-Vis . —Sigma-Aldrich (St. Louis, MO) Part No. L9879, Extrasynthese (Genay, France) Part No. 0305 S, or equivalent.

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