AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Relevant peaks include all peaks in the HPLC chromatogram with the exception of solvent peaks. Chromatographic purity is typically greater than 0.95 (i.e., 95%). (3) Reference standard purity (P) .—Calculate the purity of each reference standard: P = SP × CP × 100 where 100 = factor for converting decimal to percent. (b) Calculate the concentration of each carotenoid (e.g., lutein, C LutStock ) in the all- trans form, in μg/100 mL, in each standard stock solution, E(a) : C LutStock = (W Lut /V Stock ) × (P Lut /100) × 1000 × 100 where W Lut = the weight of lutein used to make the stock solution, in mg; V Stock = volume of the stock solution, in mL; P Lut = the reference standard purity of all- trans -lutein calculated in H(a)(3) above; 100 = conversion from percent to decimal; 1000 = conversion of mg to μg; 100 = conversion from μg/mL to μg/100 mL (c) Calculate the concentration of apocarotenal, in μg/100 mL, in the apocarotenal working solution, E(c) : C ApoWS = C ApoStock × (1/100) where C ApoStock = the concentration of apocarotenal stock solution calculated in H(b) above and (1/100) = the dilution of stock solution to working solution (d) Calculate the concentration of apocarotenal, in μg/100 mL, in the apocarotenal intermediate solution, E(d) : C ApoInterm = C ApoStock × (3/50) where C ApoStock = the concentration of apocarotenal stock solution calculated in H(b) above and (3/50) = the dilution of stock solution to intermediate solution

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