AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

(e) Calculate the concentration of each carotenoid analyte (e.g., lutein, C LutIntermed ) in the all- trans form, in μg/100 mL, in the mixed carotenoid intermediate solution, E(e) : C LutInterm = C LutStock × (V Stock /V MC ) where C Lut = the concentration of lutein stock solution calculated in H(b) above; V Stock = the volume of carotenoid stock solution, E(a) , used; V MC = the total volume of mixed carotenoid intermediate solution (f) Calculate the concentration of each carotenoid analyte (e.g., lutein, C Lut ) in the all- trans form, in μg/100 mL, in each calibration solution, E(f) : C Lut = C LutInterm × (V MC /V Total ) where C LutIntermed = the concentration of lutein in the mixed carotenoid intermediate solution calculated in H(e) above; V MC = the volume of mixed carotenoid intermediate solution, E(e) , used; V Total = dilution volume (g) Calculate the concentration of the apocarotenal internal standard (C A ), in μg/100 mL, in each calibration solution, E(f) : C Apo = C ApoInterm × (V ApoIntermed /V Total ) where C ApoIntermed = the concentration of the apocarotenal intermediate solution calculated in H(d) above; V ApoIntermed = the volume of apocarotenal intermediate solution, E(d) , used; V Total = dilution volume (h) For each calibration solution calculate ( 1 ) the peak area ratio for each analyte (peak area [all-trans lutein, β -carotene, or lycopene])/(peak area [internal standard]) and ( 2 ) the concentration ratio (concentration [all-trans lutein, β -carotene, or lycopene])/(concentration [internal standard]). Build a 5-point calibration curve with

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