AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Lut cis = (M A /M S ) × ([([A 13cisLut × 1.7] + [A 13’cisLut × 1.7] + A 9cisLut + A 9’cisLut )/A A ] – I Lut ) × (100/RF Lut ) where Lut cis = the cis isomers of lutein in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A 13cisLut = the peak area of 13- cis lutein in the sample chromatogram, in arbitrary units; A 13’cisLut = the peak area of 13′- cis lutein in the sample chromatogram, in arbitrary units; A 9cisLut = the peak area of 9- cis lutein in the sample chromatogram, in arbitrary units; A 9’cisLut = the peak area of 9′- cis lutein in the sample chromatogram, in arbitrary units; A A = the peak area of apocarotenal in the sample chromatogram, in arbitrary units; I Lut = the y-intercept of the calibration curve for all- trans -lutein; RF Lut = the slope of the calibration curve for all- trans -lutein Lut Total = Lut trans + Lut cis (k) Calculate the contents of all- trans -β-carotene, cis isomers of β-carotene, and total β- carotene on an as-is basis in the test samples. For peak identification, refer to relative retention times of peaks in Figures B – D . BC trans = (M A /M S ) × ([A BC /A A ] – I BC ) × (100/RF BC ) where BC trans = the all- trans -β-carotene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A BC = the peak area of all- trans -β-carotene in the sample chromatogram, in arbitrary units; A A = the peak area of apocarotenal in the sample chromatogram, in arbitrary units; I BC = the y- intercept of the calibration curve for all- trans - β -carotene; RF BC = the slope of the calibration curve for all- trans -β-carotene.

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