AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

BC cis = (M A /M S ) × ([([A 15cisBC × 1.4] + [A 13cisBC × 1.2] + A 9cisBC + A XcisBC )/A A ] – I BC ) × (100/RF BC )

where BC cis = the cis isomers of β-carotene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A BC = the peak area of all- trans -β-carotene in the sample chromatogram, in arbitrary units; A 15cisBC = the peak area of 15- cis -β-carotene in the sample chromatogram, in arbitrary units; A 13cisBC = the peak area of 13- cis -β-carotene in the sample chromatogram, in arbitrary units; A 9cisBC = the peak area of 9- cis -β-carotene in the sample chromatogram, in arbitrary units; A XcisBC = the peak area of unidentified cis isomers of β-carotene in the sample chromatogram, in arbitrary units; A A = the peak area of apocarotenal in the sample chromatogram, in arbitrary units; I BC = the y-intercept of the calibration curve for all- trans - β -carotene; RF BC = the slope of the calibration curve for all- trans -β-carotene. BC Total = BC trans + BC cis (l) Calculate the content of total lycopene on an as-is basis in the test samples. For peak identification, refer to relative retention times of peaks in Figure F . Lyc Total = (M A /M S ) × [(A Lyc + A XcisLyc )/A A ] – I Lyc ) × (100/RF Lyc ) where Lyc Total = the total trans and cis isomers of lycopene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A Lyc = the peak area of all- trans -lycopene in the sample chromatogram, in arbitrary units; A XcisLyc = the peak area of cis isomers of lycopene in the sample chromatogram, in arbitrary units; A A = the peak area of apocarotenal in the sample chromatogram, in arbitrary units; I Lyc = the y-intercept of the calibration curve for all- trans -lycopene; RF Lyc = the slope of the calibration curve for all- trans -lycopene.

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