AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Table C. Chromatographic conditions Analytical column

YMC C30 3 μm, 250 × 2.0 mm YMC C30 3 μm, 10 × 2.0 mm

Guard column

Column temperature 30 °C Mobile phases

A: 20 mM ammonium acetate in MeOH-Water (98+2) B: MTBE

Gradient

Time (min)

%B

0 1 8

3 8

15

25

100

25.5

3 3

32

Flow rate

0.25 mL/min ca. 185 bar

Backpressure Injection volume 5 μL UV/Visible detection 450 nm, ref = off

50