AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

( h ) Calculate the content of total lycopene in the test samples. For peak identification, refer to relative retention times of peaks in Figure 2016.13F . Lyc Total = (M A /M S ) × [(A Lyc + A XcisLyc )/A A ] – I Lyc ) × (100/RF Lyc ) where Lyc Total = total trans and cis isomers of lycopene in the sample (μg/100 g); M A = mass of apocarotenal added to the test sample, in μg; M S = sample weight, in g; A Lyc = peak area of all- trans -lycopene in the sample chromatogram (AU); A XcisLyc = peak area of cis isomers of lycopene in the sample chromatogram (AU); A A = peak area of apocarotenal in the sample chromatogram (AU); I Lyc = y -intercept of the calibration curve for all- trans -lycopene; RF Lyc = slope of the calibration curve for all- trans -lycopene. References: J. AOAC Int . 100 , 758(2017) DOI: 10.5740/jaoacint.16-0386 (First Action) AOAC SMPR 2014.014 (Carotenoids) J. AOAC Int . 98 , 1077(2015)

DOI: 10.5740/jaoac.int.SMPR2014.014 AOAC SMPR 2017.006 (β-carotene) J. AOAC Int . 100 , 1217(2017) DOI: 10.5740/jaoacint.SMPR2017.006 AOAC SMPR 2017.007 (Lutein) J. AOAC Int . 100 , 1219(2017) DOI: 10.5740/jaoacint.SMPR2017.007 AOAC SMPR 2017.008 (Lycopene) J. AOAC Int . 100 , 1221(2017) DOI: 10.5740/jaoacint.SMPR2017.008

Posted: June 2017, November 2018

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