AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Linearity

Linearity of the relative responses of analyte concentrations was measured using a 5-point standard curve on three different days. Correlation coefficients, visual inspection, residuals, and relative errors of back-calculated concentrations were used for evaluation. Linearity of the internal standard was also demonstrated. The ranges for lutein and β-carotene (4-240 μg/100 mL) correlate to approximately 0.8-45 μg/100 g as fed for samples prepared for the lowest sample concentrations. With dilutions specified in the method, the range can be extended to approximately 1500 μg/100 as fed. This extends beyond the range of 1-1300 μg/100 g for β-carotene specified in SMPR 2017.006 and 1-800 μg/100 g for lutein specified in SMPR 2017.007.

Table 1. Residuals for the internal standard Apocarotenal Conc (μg/100 mL) Residual 203.5 4.119148067 101.8 -7.399430255 40.7 -3.177165248 20.4 1.435593088 4.07 2.222225757 2.04 2.79962859

Figure 1. Linearity plot for apocarotenal.