AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 2. Residuals and error of back-calculated concentrations for all- trans -lutein standard curves. Experiment 1 Experiment 2 Experiment 3 Conc (μg/100 mL) Residual Error Residual Error Residual Error 235.9 0.000368916 0.01% -0.000497233 -0.02% -6.16301E-05 0.00% 125.8 -0.003210486 0.24% -0.00122698 0.09% 0.00221285 -0.16% 78.6 0.00379152 -0.44% 0.003308385 -0.38% -0.004609309 0.54% 31.5 0.000817163 -0.24% 0.000644834 -0.18% 0.003230669 -0.93% 3.93 -0.001767113 3.78% -0.002229005 4.65% -0.00077258 1.65%

Figure 2. Linearity plot for lutein.