AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 4. Residuals and error of back-calculated concentrations for all- trans -lycopene standard curves. Experiment 1 Experiment 2 Experiment 3 Conc (μg/100 mL) Residual Error Residual Error Residual Error 137.7 -0.001256524 -0.11% -0.005046923 -0.46% -0.001543443 -0.15% 73.4 0.0024598 0.42% 0.009183779 1.58% 0.002036853 0.37% 45.9 0.000387353 0.11% 0.001579147 0.43% 0.001259043 0.36% 18.4 -0.001354087 -0.92% -0.002418925 -1.61% 0.000571267 0.41% 2.30 -0.000236542 -1.17% -0.003297078 -14.07% -0.00232372 -11.20%

Figure 4. Linearity plot for lycopene.

LOD/LOQ

The limit of detection (LOD) and limit of quantitation (LOQ) were extrapolated from the signal-noise ratio calculated in ChemStation software when measuring analyte concentrations of 1.4-1.7 mcg/100 g. LOD was calculated as (3 × measured conc)/signal-noise ratio. LOQ was calculated as (10 × measured conc)/signal-noise ratio. Results from three different spiked samples were averaged. The LOQs for lutein, β-carotene, and lycopene meet that of ≤1 μg/100 g specified in the SMPR.