AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

β-carotene showed <1% isomerization during sample preparation. All experiments showed separation of all- trans -lutein and β-carotene from their major cis isomers, apocarotenal, α- carotene, lycopene, and zeaxanthin.

Figure 5. Lutein system suitability solution.

Figure 6. Beta-carotene system suitability solution.

Figure 7. Heat-isomerized β-carotene solution.