AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Figure 12. SPIFAN Toddler formula.

Figure 13. SPIFAN High protein adult nutritional RTF.

Figure 14. NIST SRM 1869.

Precision

All samples were prepared in triplicate and the SPIFAN samples with substantial carotenoid concentrations were prepared a at least 12 times (3 replicates × 4 days). Intermediate precision (RSD iR ) was calculated as the relative standard deviation of all replicates. Repeatability (RSD r ) for triplicate samples was calculated as the relative standard deviation, and for larger sample sizes it was estimated by performing a one-way ANOVA on the