AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 9. Precision data for lutein in SPIFAN matrices and select PN matrices (μg/100 g). For powder samples, results were calculated using a factor of 25 g powder/225 g reconstituted weight. Formula Code n cis-Lutein RSDr RSDiR trans-Lutein RSDr RSDiR Total Lutein RSDr RSDiR Child Formula Placebo 00847RF00 3 < n/a n/a < n/a n/a < n/a n/a Infant Elemental Placebo 00796RF 3 < n/a n/a < n/a n/a < n/a n/a AND RTF High Protein Placebo 00821RF00 3 < n/a n/a 3.3 3.8% n/a 3.3 3.8% n/a AND RTF High Fat Placebo 00820RF00 3 2.0 3.8 n/a 4.2 4.6% n/a 6.2 4.1% n/a IF RTF Milk Based Placebo EV4H2Q 3 < n/a n/a 0.5 5.7% n/a 0.6 4.8% n/a SRM 1849a CLC10-B 3 < n/a n/a < n/a n/a < n/a n/a IF Partially Hydrolyzed Milk Based 410057652Z 3 < n/a n/a < n/a n/a < n/a n/a IF Partially Hydrolyzed Soy Based 410057651Z 3 < n/a n/a < n/a n/a 0.3 11.5% n/a Toddler Formula Milk Based 4052755861 12 3.7 1.2% 2.2% 21.9 3.6% 3.8% 25.5 3.1% 3.4% IF Milk Based 4044755861 12 2.1 3.6% 3.7% 9.4 5.0% 5.9% 11.5 4.1% 5.1% AND Powder 00859RF00 3 < n/a n/a < n/a n/a < n/a n/a Child Formula Powder 00866RF00 3 < n/a n/a < n/a n/a < n/a n/a IF Elemental 00795RF 12 < n/a n/a < n/a n/a < n/a n/a IF w FOS/GOS 50350017W1 12 0.3 12.3% 14.3% 0.9 2.9% 5.2% 1.2 3.4% 4.2% IF Milk Based (Control) K16NTAV 39 1.6 3.3% 5.5% 4.5 1.2% 1.6% 6.1 1.6% 2.3% IF Soy Based E10NWZC 12 0.6 5.7% 8.0% 1.6 3.2% 3.4% 2.2 2.8% 3.1% IF RTF Milk Based EV4H2R 3 < n/a n/a 0.5 6.2% n/a 0.7 16.9% n/a AND RTF High Protein 00730RF00 12 1.9 4.1% 4.4% 3.1 2.4% 3.0% 4.9 2.9% 3.4% AND RTF High Fat 00729RF00 3 1.7 5.8% n/a 3.5 3.3% n/a 5.2 3.2% n/a PN AND High Lutein Powder 3 21.4 0.3% n/a 82.1 0.1% n/a 104 0.2% n/a PN Chocolate PND RTF 3 13.0 0.4% n/a 127 1.1% n/a 140 1.0% n/a PN Infant Formula RTF 3 1.5 3.8% n/a 3.9 1.4% n/a 5.5 2.0% n/a NIST SRM 1869 12 3.0 2.4% 4.4% 25.0 0.4% 0.4% 27.9 0.5% 0.7% < Below LOQ