AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 10. Precision data for β-carotene in SPIFAN matrices and select PN matrices (μg/100 g). For powder samples, results were calculated using a factor of 25 g powder/225 g reconstituted weight. Formula Code n cis-BC RSDr RSDiR trans-BC RSDr RSDiR Total BC RSDr RSDiR Child Formula Placebo 00847RF00 3 0.3 0.7% n/a 0.6 2.6% n/a 0.9 1.8% n/a Infant Elemental Placebo 00796RF 3 < n/a n/a < n/a n/a < n/a n/a AND RTF High Protein Placebo 00821RF00 3 < n/a n/a 0.8 11.2% n/a 0.8 11.2% n/a AND RTF High Fat Placebo 00820RF00 3 < n/a n/a 0.4 47.4% n/a 0.6 48.5% n/a IF RTF Milk Based Placebo EV4H2Q 3 0.5 3.3% n/a 0.4 2.2% n/a 0.9 2.6% n/a SRM 1849a CLC10-B 3 < n/a n/a < n/a n/a < n/a n/a IF Partially Hydrolyzed Milk Based 410057652Z 3 0.8 0.2% n/a 1.0 2.0% n/a 1.8 1.2% n/a IF Partially Hydrolyzed Soy Based 410057651Z 3 0.6 1.8% n/a 0.6 6.7% n/a 1.3 3.9% n/a Toddler Formula Milk Based 4052755861 12 6.5 1.0% 2.4% 7.4 0.7% 2.9% 13.9 0.8% 2.2% IF Milk Based 4044755861 12 4.4 2.1% 2.7% 10.1 1.3% 2.7% 14.4 1.5% 2.4% AND Powder 00859RF00 3 < n/a n/a 0.3 5.9% n/a 0.3 5.9% n/a Child Formula Powder 00866RF00 3 0.3 15.3% n/a 0.4 2.5% n/a 0.7 5.4% n/a IF Elemental 00795RF 12 7.3 0.3% 0.7% 15.3 0.7% 0.7% 22.7 0.5% 0.6% IF w FOS/GOS 50350017W1 12 1.3 5.1% 6.3% 3.4 3.0% 4.6% 4.8 2.3% 3.8% IF Milk Based (Control) K16NTAV 39 6.0 1.1% 2.7% 13.2 0.5% 2.2% 19.2 0.6% 1.7% IF Soy Based E10NWZC 12 6.5 2.6% 2.3% 11.0 2.0% 1.8% 17.5 2.2% 1.9% IF RTF Milk Based EV4H2R 3 0.6 6.9% n/a 0.4 4.8% n/a 1.0 4.6% n/a AND RTF High Protein 00730RF00 12 265 1.6% 2.7% 534 1.7% 2.3% 799 1.6% 2.4% AND RTF High Fat 00729RF00 3 < n/a n/a 0.3 7.7% n/a 0.4 5.9% n/a PN AND High Lutein 3 < n/a n/a 0.5 18.3% n/a 0.5 18.3% n/a PN Chocolate PND RTF 3 < n/a n/a < n/a n/a < n/a n/a PN Infant Formula RTF 3 9.1 0.3% n/a 16.4 0.4% n/a 25.5 0.4% n/a NIST SRM 1869 12 3.6 0.9% 1.1% 7.2 1.3% 1.9% 10.8 0.7% 1.4% < Below LOQ