AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Accuracy

Although SRM 1869 will be a reference material for carotenoids in infant formula and adult nutritionals, its values have not yet been certified by NIST. Accuracy was assessed by spike and recovery of all- trans lutein, β-carotene, and lycopene in various matrices. Spiked and unspiked samples were measured in triplicate. The SMPRs call for 90-110% recovery from spiked samples, and all data meet this requirement.

Table 12. Spike and recovery data for SRM 1849a. Samples were spiked with standards at the internal standard step of sample preparation. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD

Lutein

ND

1.4

105.5%

0.8%

13.8

99.6%

0.2%

β-Carotene

0.2

1.3

107.5%

0.9%

13.6

103.2%

0.3%

Lycopene

ND

1.4

103.6%

1.4%

14.0

99.8%

0.5%

ND Not detected

Table 13. Spike and recovery data for SRM 1849a. Samples were spiked with FloraGlo 5% lutein at the powder reconstitution step of sample preparation. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD Spike (μg/100 g)

Recovery RSD

Lutein

ND

34.8

92.3%

0.8%

68.3

94.0%

1.9%

135.3

97.6%

1.6%

ND Not detected

Table 14. Spike and recovery data for AND High Protein Placebo. Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD Lutein 3.3 62.7 96.7% 0.8% 148.8 100.1% 0.3% 297.6 101.0% 0.3%