AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 VALIDATION REPORT FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 15. Spike and recovery data for AND High Protein Placebo. Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Table 16. Spike and recovery data for Child Formula Placebo. Samples were spiked with standards at the internal standard step of sample preparation. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery Lutein 3.3 486.4 101.1% 0.5% 708.8 101.2% 0.5% 1425.3 Table 17. Spike and recovery data for Elemental Formula Placebo. Samples were spiked with standards at the internal standard step of sample preparation. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD Lutein ND 5.5 103.7% 0.2% 16.8 101.9% 0.6% β-Carotene 0.1 5.3 104.1% 0.2% 16.2 103.5% 0.6% Lycopene ND 3.2 102.1% 0.5% 9.8 105.1% 0.7% ND Not detected Table 18. Spike and recovery data for PN Infant Formula RTF. Samples were spiked with standards at the internal standard step of sample preparation. Native level (μg/100 g) Spike (μg/100 g) Recovery RSD Spike (μg/100 g) Recovery RSD 100.7% RSD Lutein 0.1 0.6 ND 5.5 5.3 3.3 101.8% 107.2% 100.7% 0.2% 0.2% 1.0% 16.8 16.2 100.8% 104.2% 103.7% 0.3% 0.3% 0.8% β-Carotene Lycopene 9.6 ND Not detected

Recovery RSD

0.2%

Lutein

3.9

6.2 5.9

98.7%

0.4% 0.9%

18.4 17.7

98.6%

0.1% 0.6%

β-Carotene

16.4

100.6%

100.1%