AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

B. Apparatus Note: Carotenoids are light sensitive and all work should be done under protection from UV light. (a) UHPLC System .—Consisting of binary pump, autosampler, thermostatted column compartment, UV-Vis detector with 60 mm flow cell, and data acquisition software. (b) Analytical column .—C30 Carotenoid column, 3 μm, 2.0 x 250 mm; YMC (Kyoto, Japan) Part No. CT99S03-2502WT. (c) Guard column .—C30 Guard column, 3 μm, 2.1 x 10 mm; YMC (Kyoto, Japan) Part No. CT99S03-01Q1GC. (d) Guard cartridge holder .—YMC (Kyoto, Japan) Part No. XPGCH-Q1. (e) Spectrophotometer .—Wavelength range of 200-700 nm, with 1 cm quartz cells.

(f) Top loading balance .—Capable of weighing to 0.1 g. (g) Analytical balance .—Capable of weighing to 0.01 mg. (h) Ultrasonic water bath .—40 kHz.

(i) Reciprocating shaker .—Capable of 200 rpm. (j) Evaporator .—With pure nitrogen supply. (k) Laboratory centrifuge .—With adapters for 50 mL centrifuge tubes. (l) Centrifuge tubes .—50 mL, polypropylene. (m) Syringes .—1 mL, disposable. (n) Syringe filters .—0.2 μm, PTFE. (o) Class A volumetric flasks .—Various sizes, clear and amber. (p) Scintillation vials .—12 mL, amber.

(q) HPLC vials .—amber, with 300 μL inserts. (r) Class A volumetric pipets .—Various sizes.