AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

C. Reagents Note: Reagent volumes may be scaled up or down provided good laboratory practices are followed.

(a) Laboratory water .—>18 MΩ·cm. (b) Methanol (MeOH) .—HPLC grade. (c) Methyl tert-butyl ether (MTBE) .—HPLC grade. (d) n-Hexane .—HPLC grade. (e) Potassium hydroxide (KOH) .—pellets, ACS grade. (f) Reagent alcohol (ROH) .—denatured ethanol, HPLC grade. (g) α-Tocopherol (Vitamin E) .—95%. (h) Pyrogallic acid (Pyrogallol) .—ACS grade. (i) 2-Propanol (IPA) .—HPLC grade. (j) Tetrahydrofuran (THF) .—99.9%, stabilized with BHT. (k) Ammonium acetate .—HPLC grade, 98%.

(l) Potassium hydroxide solution, 50% (w/w).— Add 50 mL water to a 250 mL beaker. Weigh 50 g KOH and slowly transfer to the beaker under constant stirring. When dissolved and cooled, transfer to a media bottle and store at room temperature up to 6 months. (m) MTBE/Vitamin E.— Dissolve 1.1 g α-tocopherol in 250 mL MTBE. Store in refrigerator up to 1 month. (n) THF/Vitamin E.— Dissolve 1.1 g α-tocopherol in 250 mL THF. Store in refrigerator up to 1 month.