AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

flask. Add ca. 40 mL THF/Vitamin E, C(n) , to each, sonicate 2-3 min, and dilute to volume with THF/Vitamin E. Store stock solutions at -20°C for up to 6 months and check their purity each time new standard solutions are made from them. (b) Standard measuring solutions for UV-visible spectroscopy potency check (200 μg/100 mL).— Transfer 1.0 mL lutein and β-carotene standard stock solutions, E(a) , to separate 100 mL volumetric flasks and dilute each to volume with MTBE. Transfer 2.0 mL lycopene standard stock solution, E(a) , to a 100 mL volumetric flask and dilute to volume with hexane. (c) Individual carotenoid working solutions for chromatographic purity check (200 μg/100 mL).— For lutein and β-carotene: Transfer 100 μL of each standard stock solution, E(a) , to separate 10 mL volumetric flasks and dilute each to volume with sample solvent. For lycopene: Transfer 200 μL standard stock solution, E(a) , to a 10 mL volumetric flask and dilute to volume with sample solvent. For apocarotenal: Transfer 1.0 mL standard stock solution, E(a) , to a 100 mL volumetric flask and dilute to volume with sample solvent. Store at -20 °C for up to one month and use for internal standard, E(i) . (d) Apocarotenal intermediate solution (1200 μg/100 mL).— Transfer 3.0 mL apocarotenal stock solution, E(a) , to a 50 mL volumetric flask and dilute to volume with sample solvent. Store at -20 °C for up to 1 month. (e) Mixed carotenoid intermediate solution (400 μg/100 mL).— Combine 2.0 mL each of lutein, β-carotene, and lycopene standard stock solutions, E(a) , in a 100 mL volumetric flask and dilute to volume with sample solvent. Store at -20°C for up to 1 month.