AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

(f) Calibration solutions.— Transfer apocarotenal intermediate solution, E(d) , and mixed carotenoid intermediate solution, E(e) , to volumetric flasks according to Table B and dilute to volume with sample solvent. Store at -20 °C for up to 1 month. (g) β-Carotene system suitability solution.— Transfer 20 mg β-carotene system suitability reference standard, D(e) , to a 50 mL volumetric flask. Add 1 mL water and 4 mL THF and sonicate 5 min. Dilute to volume with IPA and sonicate 5 min. Cool to room temperature, filter the cloudy suspension through 0.2 μm PTFE, and dilute the clear filtrate 1:4 with IPA. Store in refrigerator up to 3 months. (h) Lutein system suitability solution.— Transfer 10 mg USP lutein, D(d) , to a 100 mL volumetric flask, dilute to volume with ROH, and mix. Transfer 1 mL to a 50 mL volumetric flask. Add ca. 35 mL IPA, stopper loosely, and heat in a water bath at 80°C for two hours. Cool to room temperature, add 250 μL apocarotenal stock solution, E(a) , (1) For infant formula and samples with low carotenoid concentrations (up to 100 μg of an individual carotenoid per 100 g): Transfer 4.0 mL apocarotenal working solution, E(c) , to a 50 mL volumetric flask and dilute to volume with pyrogallol solution. This is enough solution for 9 samples. (2) For samples with individual carotenoid concentrations >100 μg/100 g: Transfer 4.0 mL apocarotenal intermediate solution, E(d) , to a 50 mL volumetric flask and dilute to volume with pyrogallol solution. F. Sample Preparation and dilute to volume with IPA. Store in refrigerator up to 3 months. (i) Internal standard solution (ISTD).— Prepare immediately before use.