AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE (AU); I Lut = the y-intercept of the calibration curve for all- trans -lutein; RF Lut = the slope of the calibration curve for all- trans -lutein Lut Total = Lut trans + Lut cis (g) Calculate the contents of all- trans -β-carotene, cis isomers of β-carotene, and total β- carotene in the test samples. For peak identification, refer to relative retention times of peaks in Figures B – D . BC trans = (M A /M S ) × ([A BC /A A ] – I BC ) × (100/RF BC ) where BC trans = the all- trans -β-carotene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A BC = the peak area of all- trans -β-carotene in the sample chromatogram (AU); A A = the peak area of apocarotenal in the sample chromatogram (AU); I BC = the y-intercept of the calibration curve for all- trans - β -carotene; RF BC = the slope of the calibration curve for all- trans -β- carotene. BC cis = (M A /M S ) × ([([A 15cisBC × 1.4] + [A 13cisBC × 1.2] + A 9cisBC + A XcisBC )/A A ] – I BC ) × (100/RF BC ) where BC cis = the cis isomers of β-carotene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A BC = the peak area of all- trans -β-carotene in the sample chromatogram (AU); A 15cisBC = the peak area of 15- cis -β-carotene in the sample chromatogram (AU); A 13cisBC = the peak area of 13- cis -β-carotene in the sample chromatogram (AU); A 9cisBC = the peak area of 9- cis -β- carotene in the sample chromatogram (AU); A XcisBC = the peak area of unidentified cis isomers of β-carotene in the sample chromatogram (AU); A A = the peak area of apocarotenal in the sample chromatogram (AU); I BC = the y-intercept of the calibration