AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE curve for all- trans - β -carotene; RF BC = the slope of the calibration curve for all- trans -β- carotene. BC Total = BC trans + BC cis (h) Calculate the content of total lycopene in the test samples. For peak identification, refer to relative retention times of peaks in Figure F . Lyc Total = (M A /M S ) × [(A Lyc + A XcisLyc )/A A ] – I Lyc ) × (100/RF Lyc ) where Lyc Total = the total trans and cis isomers of lycopene in the sample (μg/100 g); M A = the mass of apocarotenal added to the test sample, in μg; M S = the sample weight, in g; A Lyc = the peak area of all- trans -lycopene in the sample chromatogram (AU); A XcisLyc = the peak area of cis isomers of lycopene in the sample chromatogram (AU); A A = the peak area of apocarotenal in the sample chromatogram (AU); I Lyc = the y-intercept of the calibration curve for all- trans - lycopene ; RF Lyc = the slope of the calibration curve for all- trans -lycopene.