AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

Table A. Composition and nominal concentrations of carotenoid standard solutions.

Working Solutions (200 μ g/100 mL) in Sample Solvent

UV-Vis Solutions (200 μ g/100 mL)

Intermediate Solutions in Sample Solvent

Stock Solutions

Conc ( μ g/100 mL)

Standard (mg)

Total vol (mL)

Stock Solution (mL)

Total vol (mL)

Stock Solution (mL)

Total vol (mL)

Stock Solution (mL)

Total vol (mL)

Lutein

10 10

50 50 50 50

1.0 1.0 2.0

100 100 100

0.1 0.1 0.2 1.0

10 10 10

2.0 2.0 2.0 3.0

400 400 200

100

β -Carotene Lycopene Apocarotenal

5

10

-

-

100

50

1200