AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (SEPTEMBER 2017) CAROT-02 METHOD

FOR ERP USE ONLY DO NOT DISTRIBUTE

Table C. Chromatographic conditions Analytical column

YMC C30 3 μm, 250 × 2.0 mm YMC C30 3 μm, 10 × 2.0 mm

Guard column

Column temperature

30 °C

Mobile phases

A: 20 mM ammonium acetate in 98:2 MeOH/Water B: MTBE

Gradient

Time (min)

%B

0 1 8

5 8

15

25

100

25.5

5 5

32

Flow rate

0.25 mL/min ca. 185 bar

Backpressure Injection volume UV/Visible detection

5 μL

450 nm, ref = off