AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

Carot-02 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

(g) Calculate the contents of all- trans -β-carotene and cis isomers of β-carotene in the test samples:

BC trans

= RF BC

x (M A

/M S

) x (A BC

/A A

) x 100

Where BC trans

= the all- trans -β-carotene in the sample (μg/100 g)

RF BC = the response factor for all- trans -β-carotene M A = the mass of apocarotenal added to the test sample, in μg M S = the sample weight, in g A BC = the peak area of all- trans -β-carotene in the sample chromatogram (AU) A A = the peak area of apocarotenal in the sample chromatogram (AU)

BC cis

= RF BC

x (M A

/M S

) x ([(A 15cisBC

x 1.4) + (A 13cisBC

x 1.2) + A 9cisBC

+ A XcisBC

]/A A

) x 100

Where BC cis

= the cis isomers of β-carotene in the sample (μg/100 g) RF BC = the response factor for all- trans -β-carotene M A = the mass of apocarotenal added to the test sample, in μg M S = the sample weight, in g A BC = the peak area of all- trans -β-carotene in the sample chromatogram (AU) A 15cisBC = the peak area of 15- cis -β-carotene in the sample chromatogram (AU) A 13cisBC = the peak area of 13- cis -β-carotene in the sample chromatogram (AU) A 9cisBC = the peak area of 9- cis -β-carotene in the sample chromatogram (AU) A XcisBC = the peak area of unidentified cis isomers of β-carotene in the sample chromatogram (AU) A A = the peak area of apocarotenal in the sample chromatogram (AU)