AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

Carot-02 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

B. Linearity

Lutein

3000

y = 15.19x - 5.78 R² = 1.00

2500

2000

1500

1000

Peak area

500

0

0.0

50.0

100.0

150.0

200.0

Lutein (mcg/100 mL)

Beta-Carotene

5000

y = 16.79x - 2.34 R² = 1.00

4000

3000

2000

1000 Peak area

0

0.0

50.0

100.0

150.0

200.0

250.0

300.0

Beta-carotene (mcg/100 mL)

Apocarotenal

5000

y = 14.92x - 6.43 R² = 1.00

4000

3000

2000

Peak area

1000

0

0.0

50.0

100.0

150.0

200.0

250.0

300.0

Apocarotenal (mcg/100 mL)

Figure 4. Standard curves for each carotenoid analyte and the internal standard.