AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

Fluor-03 (Feb. 2019) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 7 Peak height for three individual calibration series ( fresh prepared by different analysts, analyzed with different columns at multiple days)

Standard level

1

2

3

4

5

6

7

8

9

5

10

25

50

100

250

500

1000 1.451

1500 2.260

F- (µg/kg) AVG height (µS/cm) SD height (µS/cm)

0.005

0.010

0.025

0.051

0.107

0.298

0.666

0.0004 0.0005 0.0015 0.0033 0.0060 0.0231 0.0487 0.0963 0.1378

7 3

5 3

6 3

6 3

6 3

8 3

7 3

7 3

6 3

RSD (%)

N

Figure 10 Average calibration for three individual measured calibration series with nine standard levels split into low (upper figure) and high (lower figure) calibration. The calibration standards were prepared freshly each time from different analysts and run at different days using different analytical columns. (c) Analytical quality control. —Check standards in concentrations of 5, 10, 160, and 500 μg/kg were run in duplicate for several times during the method evaluation (see Annex B and Table 8 ). The average recovery is 104±6% with RSD`s between the replicates (10-12) of 4-9%. Within the method development no QC standard drift was noticeable ( Figure 11 ).

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