AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

Fluor-03 (Feb. 2019) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 10 Reproducibility for analysis with different columns and on multiple days

No. of replicates (different columns, multiple days)

AVG c(F - ) FIN SD (µg/ 100 g)

±

RSD R (%)

Sample type

Solid infant formula SRM 1849a

14 14 12

116.5

11.7

10.1 11.8

Milumil Aptamil

62.0 36.0

7.3 1.7

4.7

"Ready-to-feed" liquids Hipp

12 12

22.4 24.7

3.2 1.7

14.3

Beba

6.7

Table 11 Repeatability precision for analysis with one column in a short time frame No. c(F - ) FIN (µg/ 100 g) AVG c(F - ) FIN ± SD (µg/ 100 g) RSD r (%) Sample ID c(F - ) FIN (µg/ 100 g)

AVG c(F - ) FIN SD (µg/ 100 g)

±

RSD r (%)

"Ready-to-feed" liquid - Hipp

Solid infant formula - SRM 1849a

1 138.3 2 139.0 3 137.9 4 138.3 5 127.0 6 N/A 7 N/A 8 N/A

136.1 5.1

3.8

1 2 3 4 5 6 7 8

18.2

0.4

2.0

18.6 17.5 18.0 18.6 18.4 18.2 18.1 18.4

(g) Accuracy. —The method accuracy was determined by spike recoveries ( Table 12) of at least two spike levels for all samples including solid infant formulas and ready-to-feed liquids. The samples and spikes were prepared and measured by different analysts and with an IC using two different columns (see Appendix C). An example for the spikes at two levels is shown in Figure 13. The recoveries for the solid formulas span a range of 80 and 105%, for the ready-to-feed liquids 103 to 108% and meet the levels of 80-120% in the SMPR® 2014.016 requirements.

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