AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

Fluor-03 (Feb. 2019) FOR ERP USE ONLY DO NOT DISTRIBUTE

Figure 13: Example for spiking of the reference material (black = native sample) at two levels (red 79 µg/100g) and green 159 µg/100g)

Table 12 Repeatability precision for analysis with one column in a short time frame

No. of replicates (different columns, multiple days)

AVG c(F - ) FIN SD (µg/ 100 g)

AVG Spike recovery (%)

±

AVG spike c(F - ) FIN ± SD (µg/ 100 g)

RSD (%)

Sample type

Solid infant formula SRM 1849a (native sample)

14

116.5 127.6 157.1 231.4

11.7 12.9 14.1

10.1 10.1

Spike level 1 Spike level 2 Spike level 3

4 8 4

38.8 79.0

0.4 0.2 0.3

82.9 80.4 84.1

9.0 2.1

4.9 7.3

158.8

Milumil (native sample) Spike level 1 Aptamil (native sample) Spike level 1 Spike level 2

14

62.0 74.3 92.6 36.0 46.1 52.3 22.4 34.5 45.4 24.7 35.5 47.2

11.8 13.7

6 6

10.2

18.9 39.3

0.3 0.2

91.9 91.4

8.7 1.7 2.8 2.5 3.2 3.0 1.9 1.7 1.6 1.1

9.4 4.7 6.2 4.7 8.7 4.1 6.7 4.4 2.3

12

6 6

7.7

0.1 0.4

105.4 101.5

Spike level 2

15.5

"Ready-to-feed" liquids Hipp (native sample)

12

14.3

Spike level 1 Spike level 2

6 6

9.5

0.1 0.3

108.0 108.2

19.5

Beba (native sample)

12

Spike level 1 Spike level 2

6 6

9.7

0.1 0.1

103.3 106.8

19.5

17 | P a g e