AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

George Joseph, Ann Daniel, and Tony Tsang, AsureQuality Limited, Auckland, New Zealand Joseph Schierle and Alexandra Schattner, DSM Nutritional Products, Basel, Switzerland

The help of Laura DePaola and Darryl Sullivan at Covance Laboratories, who assisted in shipping samples to the MLT participants, is also appreciated.

System Parameters Although the method was originally written for UHPLC systems with 60 mm flow cells, participating laboratories used a variety of LC systems. System parameters are summarized in Table 1 below. Table 1. System parameters for participating laboratories that submitted MLT data.

Flow cell path length

Lab number

LC System

Method modifications

1 2 3 4 5 6 7 8 9

Agilent 1260

60 mm 10 mm 10 mm 85 mm 10 mm 10 mm 10 mm 10 mm 10 mm 25 mm

Thermo Ultimate 3000

Shimadzu Prominance LC20

8 µL injection volume

Shimadzu Nexera X2

Agilent 1260

Used 2.0 x 150 mm column, modified gradient

Shimadzu LC-10AD modified

Agilent 1200 Agilent 1260

Waters Acquity UPLC

10 µL injection volume 15 µL injection volume

10

Waters Acquity UPLC with TUV

5