AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Standard Purity and Linearity Participating laboratories sourced standards from various manufacturers and tested them for purity according to the method. The purity of standards and the response factors (calibration curve slopes) are summarized in Table 2 below. Table 2. Purity and response factors of standards used by participating laboratories that submitted MLT data. Lutein β-Carotene Lycopene

Avg response factor (slope)

Avg response factor (slope)

Avg response factor (slope)

UV/Vis purity, %

LC Purity, % 90.66 92.55 98.98 95.52 98.10 91.93 92.22 89.41 90.51 92.06

Total purity, % 73.49 69.00 95.06 58.16 94.84 99.79 72.13 85.27 100.51

UV/Vis purity, % 102.54 113.57 103.34 100.03 103.14 96.23

LC Purity, % 96.97 97.22 95.37 95.14 95.67 96.29 95.87 95.93 97.21 95.45

Total purity, % 99.43 93.55 108.31

UV/Vis purity, %

LC Purity, % 95.83 98.50 80.51 94.04 92.60 91.74 87.62 93.75 99.22 80.69

Total purity, % 76.98 73.37 98.88 94.91 71.23 99.43 65.77 94.35 78.66 68.31

Lab number

1 2 3 4 5 6 7 8 9

81.06 74.56 96.04 60.89 96.67

1.048 1.019 0.985 0.821 1.162 1.019 1.122 1.089 1.099 1.161

1.113 1.067 1.028 0.940 1.210 1.100 1.671 1.087 1.098 1.017

80.33 74.49

0.863 0.874 0.885 0.708 0.918 0.641 0.886 1.070 0.871 0.700

122.83 100.93

98.32 96.60 99.31 75.32 94.82 96.08

76.92

108.54

108.39

78.22 95.37

78.57 98.85 98.83

75.07

100.63

111.05

79.28 84.65

10

97.05

89.34

107.12

102.25

All 10 participating laboratories provided acceptable calibration data based on the coefficient of determination: R 2 ≥0.9991 for lutein, ≥0.9997 for β-carotene, and ≥0.9990 for lycopene.

6