AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Lutein Precision Raw data for all- trans -lutein and total lutein is shown in Tables 3 and 4. After outliers were removed, the repeatability for all- trans -lutein ranged from 1.5 to 10.0% RSD and repeatability for total lutein ranged from 2.2 to 12.0% RSD (Tables 5 and 6). The repeatability values met the criteria in the SMPR for lutein with the exception of milk-based infant formula. Reproducibility for all- trans -lutein ranged from 10.5 to 14.8% RSD and for total lutein it ranged from 11.5 to 19.9% RSD. Although the all- trans -lutein data met the reproducibility limit for the SMPR, the data for total lutein data was above the SMPR limit for most matrices. The Horwitz ratio (HorRat) is used to assess reproducibility data relative to predicted method reproducibility based on historical performance of methods at the same analyte concentration. HorRat values in the range of 0.5-1.5 indicate normally expected reproducibility, while HorRat values above 2.0 indicate poor reproducibility. Figures 7 and 8 show that all HorRat values for all- trans -lutein and total lutein are below 1.0, so the method reproducibility is better than predicted. Table 5. Precision data for all- trans -lutein

Precision parameter

Symbol

A

B

C

D

E

F

Total number of laboratories Total number of replicates

p n

10 10

10 10

10

10 10

10

10

9 1

9 1

9 1

Number of outliers

-

0

0

0

Grand mean (µg/100 g) Repeatability SD (µg/100 g) Reproducibility SD (µg/100 g) Repeatability RSD (%) SMPR repeatability limit Reproducibility RSD (%) SMPR reproducibility limit

x̅

24.6

25.0

72.0

9.7 1.0 1.1

4.7 0.1 0.5 2.3

123.1

SD r SD R

0.6 3.5 2.4

2.0 3.7 8.0

1.1 7.8 1.5

3.5

16.5

RSD r

10.0

2.8

-

8

8

8

8

8

5

RSD R

14.0

14.8

10.9

10.9

10.5

13.4

-

15

15

15

15

15

10

Horwitz ratio 0.86 Sample A = NIST SRM 1869, B = Milk-based toddler formula powder, C = Adult nutritional powder, D = Milk-based infant formula powder, E = Milk-based infant formula powder, F = Chocolate pediatric nutritional ready-to-feed HorRat 0.71 0.75 0.65 0.48 0.41

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