AOAC SPIFAN Nutrients ERP Review (March 14, 2019)

2016.13 (Jan. 2019) Carot-02 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Lycopene Precision Raw data for total lycopene in SRM 1869 is shown in Table 11. After outliers were removed, the repeatability for total lycopene was 1.6% RSD and reproducibility was 7.4% RSD (Table 12). The repeatability and reproducibility values met the criteria in the SMPR for total lycopene. Table 12. Precision data for total lycopene in fortified formula

Precision parameter

Symbol

A

Total number of laboratories Total number of replicates

p n

10

8 2

Number of outliers

-

Grand mean (µg/100 g) Repeatability SD (µg/100 g) Reproducibility SD (µg/100 g) Repeatability RSD (%) SMPR repeatability limit Reproducibility RSD (%) SMPR reproducibility limit

x̅

30.1

SD r SD R

0.5 2.2 1.6

RSD r

-

8

RSD R

7.4

-

15

Horwitz ratio

HorRat

0.39

Sample A = NIST SRM 1869

Accuracy NIST SRM 1869 was used as a reference material for carotenoids in infant formula and adult nutritionals. The SMPRs call for 90-110% recovery, and all data meet this requirement (Table 13). Table 13. Method accuracy for carotenoids in NIST SRM 1869

Precision parameter

Symbol Lutein β-Carotene Lycopene

Total number of replicates Grand mean (mg/kg) Reference value (mg/kg)

n x̅ x

10

9

8

2.82

0.98 1.05 0.26 93%

2.71 2.47 0.23

2.6 1.5

Uncertainty

U 95%

(x)

Recovery

108%

110%

-

Concentrations on powder basis

24