AOAC SPIFAN Nutrients ERP Reviewer Forms (Amino-05)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

Pros/Strengths: Well-known analytical approach using Ninhydrin reagent for analysis of amino acid. The same chromatographic conditions can be used to determine all the amino acids listed in SMPR but sample hydrolysis step is needed if analysis of total amino acids is required

Cons/Weaknesses The exact composition of buffers and reagents is not available so all the solutions have to be purchased from the manufacturer of the amino acids analyzer.

Long run time. If no other amino acids besides Taurine and Norleucine are present on the chromatogram it would be beneficial to optimize the method for shorter run time.

Supporting Data • General Comment:

Method Optimization:

• Performance Characteristics:

Analytical Range : 0.5 – 2500 mg/100 g for liquids or reconstituted powders – meets SMPR

LOQ: 0.5 mg/100g for reconstituted powder samples – meets SMPR

Accuracy/Recovery: meets SMPR – 2 samples with 3 spike levels were used for accuracy study

Precision (RSD r ): meets SMPR – 5 samples used for precision study

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