AOAC SPIFAN Nutrients ERP Reviewer Forms (December 7, 2022)
FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION
Expert Review Panel for Infant Formula and Adult Nutrition Evaluation of Method Amino-07
Title: Analysis of Taurine in Infant Formula and Adult Nutritionals by Hydrophilic Interaction Liquid Chromatography-Tandem Mass
Spectrometry (HILIC-MS/MS) Author: Brendon D. Gill, Fonterra Reviewer Name: Reviewer 1
Summary of Method: Following protein precipitation with Carrez solution, taurine is extracted and separated by hydrophilic interaction liquid chromatography (HILIC) with detection by tandem mass spectrometry (MS/MS) using multiple reaction monitoring (MRM). Stable isotope labelled (SIL) taurine internal standard is used for quantitation to correct for losses in extraction and any variation in ionization
Method Scope/Applicability: The method is applicable to Taurine in Infant Formula and Adult Nutritionals
General comments about the method: The method is scientifically sound and utilizes commonly available instrumentation and materials. The method undergone comprehensive single laboratory validation using 12 samples that included a range of samples from SPIFAN II kit, commercial products and NIST SRMs. The method was shown to meet SMPR.
Method Clarity: Method is clearly written, full information about instrumentation, chemicals and supplies is provided. All steps of the analysis are clearly outlined.
Pros/Strengths: • Fast analysis
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