AOAC SPIFAN Nutrients ERP Reviewer Forms (December 7, 2022)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

• Uses common instrumentation and chemicals • Uses isotope labelled internal standard

Cons/Weaknesses • Only applicable to Taurine so a different method needs to be used for other amino acids

Supporting Data • General Comment:

SLV evaluated performance characteristics outlined in SMPR as well as additional parameters such as method ruggedness. All data are clearly presented in the SLV report. Comparison with currently approved AOAC method 997.05 showed good agreement in results.

Method Optimization:

• Performance Characteristics:

Analytical Range: 0.27–2700 mg/100 g (meets SMPR)

LOQ: 0.14 mg/100 g (meets SMPR)

Accuracy/Recovery: 97.2–100.1 % (meets SMPR). Precision (RSD r ): 1.6–6.4 % (meets SMPR)

Reproducibility (RSD R ):

2