AOAC SPIFAN Nutrients ERP Reviewer Forms (December 7, 2022)
FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION
• Performance Characteristics:
Analytical Range : SMPR: 0.5–2500 mg/hg Method: 0.27–2700 mg LOQ: SMPR: ≤ 0.5 mg /hg Method: 0.14 mg/hg
The LoD was estimated from 10 replicate analyses of an adult nutritional formula with a low concentration of taurine. The LoD was calculated as 0.04 mg/hg RTF and the LoQ is 0.14 m/hg RTF. Accuracy/Recovery: SMPR: 0.5-5.0 mg/hg 88-112%
5.0-150 mg/hg 90-110% 150-2500 mg/hg 93-107%
Method: 0.5-5.0 mg/hg 99.2% 5.0-150 mg/hg 102.3% 150-2500 mg/hg 101.4%
-Two certified reference materials, NIST 1849a, NIST 1869, were analized to evaluate the accuracy. The calculated p-values for both NIST 1949a and NIST 1869 indicate that no bias between the reference values and the measured values by the candidate method could be found. Recovery: Recovery was evaluated using an adult nutritional formula with a low concentration of taurine. Each replicate was spiked at three concentration levels: low (0.7 mg/hg), medium (7 mg/hg), and high (70 mg/hg). Spiked and unspiked samples were analysed in triplicate on three separate days.
Average method recoveries were estimated from 97.2–100.1 %
Precision (RSD r ): SMPR: 0.5-5.0 mg/hg
≤7% ≤5% ≤3%
5.0-150 mg/hg 150-2500 mg/hg
Method: 0.5-5.0 mg/hg 4.5% 5.0-150 mg/hg 1.8% 150-2500 mg/hg 1.8%
Precision as repeatability and intermediate precision was assessed by testing duplicate of 7 samples of different products on six separate days (5 SPIFAN samples, NIST SRM 1859 and NIST SRM 1869). The repeatability for the method ranged between 1.6–6.4 % RSDr.
2
Made with FlippingBook flipbook maker