AOAC SPIFAN Nutrients ERP Reviewer Forms (December 7, 2022)

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• Performance Characteristics:

Analytical Range : SMPR: 0.5–2500 mg/hg Method: 0.27–2700 mg LOQ: SMPR: ≤ 0.5 mg /hg Method: 0.14 mg/hg

The LoD was estimated from 10 replicate analyses of an adult nutritional formula with a low concentration of taurine. The LoD was calculated as 0.04 mg/hg RTF and the LoQ is 0.14 m/hg RTF. Accuracy/Recovery: SMPR: 0.5-5.0 mg/hg 88-112%

5.0-150 mg/hg 90-110% 150-2500 mg/hg 93-107%

Method: 0.5-5.0 mg/hg 99.2% 5.0-150 mg/hg 102.3% 150-2500 mg/hg 101.4%

-Two certified reference materials, NIST 1849a, NIST 1869, were analized to evaluate the accuracy. The calculated p-values for both NIST 1949a and NIST 1869 indicate that no bias between the reference values and the measured values by the candidate method could be found. Recovery: Recovery was evaluated using an adult nutritional formula with a low concentration of taurine. Each replicate was spiked at three concentration levels: low (0.7 mg/hg), medium (7 mg/hg), and high (70 mg/hg). Spiked and unspiked samples were analysed in triplicate on three separate days.

Average method recoveries were estimated from 97.2–100.1 %

Precision (RSD r ): SMPR: 0.5-5.0 mg/hg

≤7% ≤5% ≤3%

5.0-150 mg/hg 150-2500 mg/hg

Method: 0.5-5.0 mg/hg 4.5% 5.0-150 mg/hg 1.8% 150-2500 mg/hg 1.8%

Precision as repeatability and intermediate precision was assessed by testing duplicate of 7 samples of different products on six separate days (5 SPIFAN samples, NIST SRM 1859 and NIST SRM 1869). The repeatability for the method ranged between 1.6–6.4 % RSDr.

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