AOAC SPIFAN Nutrients ERP Reviewer Forms (July 28, 2022)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

The NIST SRM 1869 was analized through spikes at two ranges in the middle/high field of SMPR. Spiked and unspiked samples were analyzed in triplicate on each three days. Results: 99.6 % ±12% for low Taurine content (0.5-5.0)

96.8 % ±3.2% for medium Taurine content (5.0-150) 98.4 % ±3.1% for high Taurine content (150-2500)

Precision (RSD r ): SMPR: 0.5–5.0

≤7%

5.0–150 ≤5% 150–2500 ≤3%

The precision was assessed by repeated measurements carried out on 6 different days, in duplicate on the each sample of SPIFAN kit. The precision was evaluated also on spiked samples NIST 1869 and NIST 8261 to cover all the analytical range. Results:

0.5–5.0 1.4% 5.0-150 2.0 % 150-2500 0.9% Reproducibility (RSD R ): No data

• System suitability:

Blank acceptance criteria No peaks detected except that of Norleucine internal standard.

Acceptance of the Calibration curve The calibration curve is considered acceptable if the Linear Regression coefficient is ≥ 0.995 .

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