AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

A.

SAFETY LABORATORY CHEMICALS SHOULD BE HANDLED WITH THE PRECAUTIONS, CARE AND RESPECT THAT ALL POTENTIALLY HAZARDOUS MATERIALS REQUIRE. AVOID SKIN CONTACT AND INJESTION OF ALL COMPOUNDS, REAGENTS AND SOLUTIONS USED IN THIS PROCEDURE. MOST REAGENTS USED IN THIS METHOD POSE MINIMAL HEALTH AND/OR FIRE HAZARDS. THOROUGHLY FLUSH ANY AREA COMING IN CONTACT WITH ANY REAGENTS USED IN THIS METHOD WITH WATER. IN GENERAL, DILUTE SOLUTIONS PRODUCED TO SUPPORT THE PREPARATION OF SAMPLES FOR ANALYSIS, MOBILE PHASE COMPONENTS AND SAMPLE EXCESS SAMPLE EXTRACT VOLUMES CAN BE DISCARDED DOWN THE DRAIN. WASTES GENERATED SHOULD BE DISCARDED IN ACCORD WITH ALL CORPORATE, LOCAL AND FEDERAL REGULATIONS. THROUGHOUT THIS DOCUMENT STEPS REQUIRING SPECIFIC SAFETY PRECAUTIONS OR PERSONAL PROTECTIVE EQUIPMENT ARE DENOTED BY THE FOLLOWING SYMBOLS:

USE OF GLOVES IS REQUIRED. LATEX, NITRILE OR RUBBER GLOVES ARE RECOMMENDED.

PERFORM THIS PROCEDURAL STEP USING A WORKING FUME HOOD. THE CHEMICALS USED IN THIS STEP REPRESENT AN INHALATION HAZARD.

1. METHANOL

TOXIC! CAN CAUSE BLINDNESS. CUMULATIVE POISON. HARMFUL IF INHALED, SWALLOWED, OR ABSORBED THROUGH SKIN. USE WITH ADEQUATE VENTILATION AND AVOID BREATHING VAPORS. AVOID CONTACT WITH SKIN OR EYES.

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