AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

Thiamin Riboflavin

250 800

QS to the mark with laboratory water. Expiration: Prepare fresh daily.

i) Working Standard Solutions NOTE: As a general rule, use the Gilson pipette with the smallest maximum volume to dispense the specified volumes of IWSS and ISSM solutions. Combine the IWSS and ISSM solution volumes shown in Table 8 into a 50-mL self-standing centrifuge tube. Pipette in 5 mL of enzyme solution, and then add 50mM Ammonium Formate up to a final volume of 30mL. Expiration: Prepare fresh daily.

Table 5. Working Standard solution preparation.

IWSS Volume (µL)

ISSM Volume (µL)

Final Volume (mL)

Gilson Pipette (recommended)

Gilson Pipette (recommended)

Solution

WS 1 WS 2 WS 3 WS 4 WS 5 WS 6

25 50

M25 M100 M100 M250 M1000 M1000

50 50 50 50 50 50

M100 M100 M100 M100 M100 M100

30 30 30 30 30 30

100 250 500

1000

.

NOTE: The accurate and precise addition of the IWSS and the ISSM solutions are critical to the accuracy and precision of the method. Extreme care must be taken in aliquoting the IWSS into the standards and the ISSM into the standards and the samples. NOTE: Due to the addition of internal standards for each compound the final volume of the working standards is not a critical volume. Small errors (on the order of approximately 1-2 mL) in the final volume will not adversely affect the calibration accuracy.

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