AOAC SPIFAN Stakeholder Panel (August 25, 2018)

Microbiological Method for B12 Challenges faced in the microbiological method: 1. Tedious 2. Maintenance of the bacterial culture (contamination is a big issue) 3. Interference from other food components 4. Requirement of skilled manpower to handle and maintain the culture and the analysis

AOAC INTERNATIONAL methods

AOAC 952.20 Vitamin B12 in vitamin preparations - Microbiological assay

AOAC 986.23 Vitamin B12 in milk-based infant formula - Microbiological assay

AOAC 2014.02 (old - 2011.08) - HPLC with Immuno- affinity extraction (Final action) Only SLV data available