AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Vitamin E Approved Methods

AOAC Official Method 992.03 Vitamin E Activity (All-rac-a-Tocopherol) in Milk-Based Infant Formula Liquid Chromatographic Method

AOAC Official Method 2012.10 Simultaneous Determination of Vitamins E and A in Infant Formula and Adult Nutritionals Normal-Phase High-Performance Liquid Chromatography

AOAC Official Method 2012.09 Vitamins A and E in Infant Formula and Adult/Pediatric Nutritional Formula HPLC with UV and Fluorescence Detection

Summary of Existing Methods for Vitamin E in OMA, Chapter 50

AOAC Official Method 992.03 Vitamin E Activity (All-rac-a-Tocopherol) in Milk-Based Infant Formula

Analytes: -tocopherol

Standard: -tocopherol, -tocopherol acetate

Prep: Saponification and solvent extraction

Normal-phase, Si, 5um

MP: Hexane–isopropyl alcohol (99.92 + 0.08, v/v), Flow = 2.0 ml/min

Detection: UV 280nm

Mean recovery = 100.4%; RSDr = 8.46%; RSDR = 11.69%, Range = na

Limitations: 280nm; 100ul inj; 40um C18 guard, 2.0 ml/min flow