AOAC SPIFAN Stakeholder Panel Meeting Book (March 14, 2019)

Current SMPR – AOAC 2014.013

Revision Proposed in March 2018

SPIFAN requests further consideration of RSD r and RSD R

ERP recommendation for reconsideration of RSD r and RSD R in SMPR

Working Group Recommended Revised Method Performance Requirements – July 2018

Footnotes: Concentrations apply to: a) ‘ready-to- feed” liquids “as is”; b) re-constituted powders (25 g into 200 g of water); and c) liquid concentrates diluted 1:1 by weight using water. The results from the following compounds may fall slightly outside of the performance parameters due to the diverse nature of amino acids regarding the degradation, conversion, and/or hydrolysis: Threonine, Serine, Tyrosine, Methionine, Cystine/Cysteine, Valine, and Isoleucine. *mg /100 g reconstituted final product.

Method Performance Requirements:

Analytical range

0.5-2500*

Limit of Quantitation (LOQ)

ч Ϭ͘ϱΎ

Recovery

0.5 – 5.0* 5.0 – 150 150 – 2500 0.5 – 5.0* 5.0 – 150 150 – 2500 0.5 – 5.0* 5.0 – 150 150 – 2500

± 12% + 10% + 7%

Repeatability (RSD r )

ч 7% ч 5% ч 3%

Reproducibility (RSD R )

ч 11% ч 8% ч 5%