AOAC SPIFAN Stakeholder Panel Meeting Book (March 14, 2019)

Responses to Comment (PH cont.) • Depending on how testing is implemented there can be a difference in testing results for free versus total taurine due to Schiff base formation in some products. A mild acid hydrolysis can liberate the taurine to return the value back closer to what is expected from fortification levels (this is outside of the scope of the SMPR currently though). • Hydrolysis of some bile acids yields taurine (i.e.., taurocholic acid). This is likely out of scope for SPIFAN though I think… • Some of Abbott’s internal data indicates that acid hydrolysis regularly yields >100% recovery of what we fortify in as a free form.

Responses to Comment (PF) I agree with Phil’s comment about tryptophan analysis.

I do not have enough knowledge about the nutritional value corresponding to the taurine status. Based on my previous experience, we have reported taurine value after acid hydrolysis, but it seems the nutritional value of taurine is expressed under free status in all the literature. In the latest SMPR, we didn’t point out that the taurine should be Ā determined only as free amino acids”, that implies that taurine value should include free and bonded.